[Repairing bone defect with autogenous periosteal pieces in coagulum: experimental study and clinical application]

A 1.5 cm-long bony defect was established in rabbits one each on both sides. The defected area was filled with coagulum of peripheral blood or bone marrow blood, then autogenous pieces of the periosteum was put in the coagulum on one side, while the other side was served as the control without periosteal autograft. The osteogenetic capacity of the periosteum and the influence of different blood coagulum were evaluated by means of X-ray film, histology, the contents of calcium and phosphate in new osteocytes in the defected area, bone tissue morphometry, and computer-assisted image analysis. The autograft of the periosteum had osteogenetic effect. Autologous bone marrow blood had potential osteogenetic capacity, which however, was weaker than that of the periosteum. Peripheral blood coagulum did not have any osteogenetic effect. We have successfully used the periosteum-bone marrow blood complex (bone marrow blood as a carrier) in 12 patients.