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Related Experiment Videos

DNA content differences in laboratory mouse strains determined by flow cytometry

R Capparelli1, C Cottone, L D'Apice

  • 1University of Naples Federico II, ENEA Centro Ricerche, Casaccia, Rome.

Cytometry
|December 6, 1997
PubMed
Summary

Nuclear DNA content varies significantly between mouse strains and sexes. This finding enables studying genome size variation in mice and offers a rapid method for mouse strain identification.

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Area of Science:

  • Genomics
  • Comparative Genomics
  • Flow Cytometry

Background:

  • Genome size variation is a fundamental aspect of biology, yet its adaptive significance is primarily studied in plants.
  • Understanding genome size variation in mammals, like laboratory mice, can provide insights into evolutionary processes and genetic diversity.

Purpose of the Study:

  • To quantify and compare the nuclear DNA content across seven different mouse laboratory strains.
  • To investigate the statistical significance of variations in DNA content between strains and between sexes within strains.
  • To explore the potential of DNA content analysis as a tool for mouse strain identification and as a model for studying genome size variation.

Main Methods:

  • Utilizing flow cytometry to accurately measure the nuclear DNA content in cells from seven distinct mouse laboratory strains.

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  • Performing statistical analyses to determine the significance of observed differences in DNA content between strains and sexes.

    • Main Results:

    • Statistically significant differences in nuclear DNA content were observed both between the seven mouse strains and between male and female individuals within each strain.
    • The Balb/c strain exhibited the highest DNA content (approx. 6.4 pg/female nucleus), while the C3H/he strain showed the lowest (approx. 5.7 pg/male nucleus).
    • Sex-based differences in DNA content ranged from 1.6% in CD-1 mice to 6.3% in nude mice.

    Conclusions:

    • The mouse, with its quantifiable DNA content variations, can serve as a valuable model organism for studying the adaptive significance of genome size variation, extending this research beyond plants.
    • DNA content analysis via flow cytometry presents a rapid and reliable method for identifying specific mouse strains, aiding in research consistency and quality control.