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Permeable packings and perfusion chromatography in protein separation
1Laboratory of Separation and Reaction Engineering, Faculty of Engineering, University of Porto, Portugal.
Journal of Chromatography. B, Biomedical Sciences and Applications
|December 10, 1997
Summary
Perfusion chromatography uses permeable packings for efficient protein separation by enhancing mass transport. This method improves chromatographic column performance over conventional supports.
Area of Science:
- Chromatography
- Bioseparations
- Chemical Engineering
Background:
- Perfusion chromatography utilizes permeable packings for protein separation.
- Mass transport in these materials involves both diffusion and forced convection.
- Conventional chromatography supports rely primarily on diffusion.
Purpose of the Study:
- To review the use of permeable packings in perfusion chromatography.
- To explain the enhanced efficiency of perfusive packings.
- To propose a methodology for understanding mass transfer in permeable packings.
Main Methods:
- Review of mass transport mechanisms in large-pore materials.
- Application of an extended Van Deemter equation for perfusive columns.
- Analysis of experimental results for protein separation using high-performance liquid chromatography (HPLC) and new packing media.
- Discussion of simulated moving bed (SMB) technology.
Main Results:
- Perfusion chromatography demonstrates improved efficiency due to 'augmented' diffusivity from convection.
- Forced convective flow in pores influences separation performance, bridging diffusion-controlled and equilibrium limits.
- Experimental data validates the effectiveness of new packing media in protein separation via HPLC.
Conclusions:
- Permeable packings in perfusion chromatography offer significant advantages for protein separation.
- Understanding augmented diffusivity is key to optimizing chromatographic performance.
- The proposed methodology aids in analyzing mass transfer in advanced chromatographic systems.