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High affinity human antibodies by phage display
L Aujame1, F Geoffroy, R Sodoyer
1Department of Molecular Biology, Pasteur Mérieux Connaught, Marcy l'Etoile, France.
Human Antibodies
|January 1, 1997
Summary
Phage display enables direct isolation of human antibodies without immunization. Complex
Area of Science:
- Immunology
- Biotechnology
Background:
- Phage display technology has advanced significantly.
- Human immunogenetics has seen recent breakthroughs.
Purpose of the Study:
- To isolate human antibodies without immunization using phage display.
- To assemble and screen complex naive human antibody repertoires in vitro.
Main Methods:
- Assembly of naive human repertoires using phage technology.
- Screening of these repertoires to identify specific antibodies.
Main Results:
- Isolation of human antibodies in a single step.
- Achieved nanomolar affinity range for isolated antibodies.
Conclusions:
- Phage display is a viable method for direct human antibody isolation.
- In vitro assembled repertoires mimic natural immune system complexity and function.