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Updated: Aug 6, 2026

Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Detection of a single base exchange in PCR-amplified DNA fragments using agarose gel electrophoresis containing
M Müller1, L Kruse, A M Tabrett
1Hanse Analytik GmbH, Fahrenheitstrasse 1, D-28359 Bremen, Germany. m.mueller@hanse-analytik.de
Abstract:
Using PCR fragments of known sequences derived from isolates of two related fungal species, simple submarine electrophoresis in agarose gels containing a bisbenzimide-PEG conjugate (H.A.-Yellow) has been shown to be capable of distinguishing DNA fragments 567 bp long which differ by as little as a single base change. However, only changes affecting bisbenzimide binding sites (which consist of at least four consecutive A/T bases) alter mobility; other changes are ineffective. A second ligand (H.A.-Red) with high G/C specificity is suggested which may be as effective in detecting other sequence changes.
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