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Study of pathogenicity markers of staphylococci isolated from clinical specimens
Abstract:
A total of 165 strains of coagulase positive staphylococci (CPS) and 39 strains of coagulase negative Staphylococci (CNS) isolated from various clinical specimens studied for biochemical and enzyme profile showed overlapping of the key characters of pathogenicity. Anaerobic mannitol fermentation (69.20%), phosphatase (58.97%) and penicillinase (58.97%) production was remarkable amongst CNS. Both CPS and CNS showed increased resistance to penicillin and other antimicrobials. Besides increased frequency of isolation of CNS from pathological specimens, they elaborate singly or in combination, the recognized virulence factors.
Insights
Coagulase-negative Staphylococci (CNS) exhibit significant pathogenicity, with notable anaerobic mannitol fermentation and enzyme production. Both CNS and coagulase-positive Staphylococci (CPS) demonstrate increasing antimicrobial resistance, highlighting evolving infectious threats.
Area of Science:
- Microbiology
- Clinical Bacteriology
- Infectious Diseases
Background:
- Staphylococci are significant human pathogens.
- Coagulase-positive Staphylococci (CPS) and coagulase-negative Staphylococci (CNS) are common causes of infection.
- Understanding their pathogenic potential is crucial for effective treatment.
Purpose of the Study:
- To investigate the biochemical and enzyme profiles of clinical Staphylococci isolates.
- To compare the pathogenicity markers between CPS and CNS.
- To assess antimicrobial resistance patterns in these strains.
Main Methods:
- Isolation and identification of Staphylococci strains from clinical specimens.
- Biochemical characterization including anaerobic mannitol fermentation.
- Enzyme profiling for phosphatase and penicillinase production.
- Antimicrobial susceptibility testing.
Main Results:
- Overlapping pathogenicity markers were observed between CPS and CNS.
- CNS showed remarkable anaerobic mannitol fermentation (69.20%), phosphatase (58.97%), and penicillinase (58.97%) production.
- Both CPS and CNS exhibited increased resistance to penicillin and other antimicrobials.
- CNS were frequently isolated from pathological specimens and produced virulence factors.
Conclusions:
- CNS possess significant pathogenic potential, comparable to CPS in some aspects.
- The increasing antimicrobial resistance in both CPS and CNS necessitates updated treatment strategies.
- Further research into CNS virulence factors is warranted.