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DNA amplification polymorphisms of Mucor piriformis
T Papp1, C Vágvölgyi, Z Kerényi
1Department of Microbiology, Attila József University, Szeged, Hungary.
Antonie Van Leeuwenhoek
|December 24, 1997
Summary
Random amplified polymorphic DNA (RAPD) analysis revealed significant genetic diversity within Mucor piriformis, a fungus causing postharvest decay. This molecular technique effectively identified distinct fungal populations, aiding in understanding disease spread and management.
Area of Science:
- Mycology
- Plant Pathology
- Molecular Biology
Background:
- Mucor piriformis is a fungal pathogen responsible for significant postharvest decay in fruits and vegetables, particularly under refrigerated storage conditions.
- Understanding the genetic diversity of M. piriformis is crucial for developing effective disease management strategies and preventing economic losses in the agricultural sector.
Purpose of the Study:
- To investigate the genetic variability within and among different isolates of Mucor piriformis.
- To assess the utility of random amplified polymorphic DNA (RAPD) analysis for differentiating M. piriformis isolates and identifying subspecific populations.
Main Methods:
- Thirty isolates of Mucor piriformis, obtained from infected produce, were analyzed using random amplified polymorphic DNA (RAPD) markers.
- Seven distinct 10-bp primers were employed to amplify genomic DNA, generating polymorphic banding patterns.
- Numerical analysis, including Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering, was performed on the RAPD data.
Main Results:
- Nineteen unique composite amplification types were identified among the 30 isolates, indicating a high level of intraspecific genetic polymorphism.
- The UPGMA analysis delineated three distinct clusters of isolates.
- These genetic clusters showed a correlation with the mating competency and geographical origin of the Mucor piriformis isolates.
Conclusions:
- Random amplified polymorphic DNA (RAPD) analysis is a powerful and sensitive molecular tool for identifying and differentiating isolates of Mucor piriformis.
- The study revealed greater genetic diversity in M. piriformis than previously observed using isoenzyme analysis.
- The identified genetic clusters provide insights into the population structure of M. piriformis, potentially aiding in targeted disease control measures.