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Synthesis of asymmetrically labeled sucrose by a recombinant sucrose synthase
T Nakai1, N Tonouchi, T Tsuchida
1Wood Research Institute, Kyoto University, Japan.
Bioscience, Biotechnology, and Biochemistry
|December 24, 1997
Abstract:
About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[14C]glucose or [14C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.