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Updated: Aug 5, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Published on: July 9, 2021
ROESY with water flip back for high-field NMR of biomolecules
1Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec, H4P 2R2, Canada.
Abstract:
We report a version of the ROESY experiment in which saturation of the water magnetization is avoided without compromising suppression of the water signal during acquisition. Field gradient and selective RF pulses are used to maintain precise control of the water magnetization throughout the experiment and avoid signal losses due to radiation damping and molecular diffusion effects. The pulse sequence includes a delay for intentional radiation damping prior to mixing period. The optimal length of this delay is field and sample dependent, but easily determined from the apparent linewidth of the water signal. NOESY and TOCSY variants of the same experiment are presented which make use of identical manipulations of the water magnetization. The three pulse sequences constitute a suite for which little parameter adjustment is required once one of the experiments has been configured.
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