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Updated: Aug 10, 2026

Assays for the Degradation of Misfolded Proteins in Cells
Published on: August 28, 2016
Degradative covalent reactions important to protein stability
D B Volkin1, H Mach, C R Middaugh
1Merck Research Laboratories, West Point, PA 19486, USA.
Abstract:
Commonly observed chemical modifications that occur in proteins during their in vitro purification, storage, and handling are discussed. Covalent modifications described include deamidation and isoaspartate formation, cleavage of peptide bonds at aspartic acid residues, cystine destruction and thiol-disulfide interchange, oxidation of cysteine and methionine residues, and the glycation and carbamylation of amino groups.
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