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Purification of bovine alpha-lactalbumin by immobilized metal ion affinity chromatography

A L Blomkalns1, M R Gomez

  • 1Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA.

Preparative Biochemistry & Biotechnology
|December 31, 1997
PubMed

The milk protein alpha-lactalbumin was isolated from bovine whey protein concentrate solution by immobilized metal ion affinity chromatography (IMAC) using Cu(II)-Chelating Sepharose Fast Flow. Stepwise pH (5.5-3.8) changes in sodium acetate buffer were used to elute the protein selectively, at which time it was concentrated and reapplied to an uncharged Chelating Sepharose Fast Flow column to remove the contaminating Cu(II) ions. A purity of 90% and recovery of 80% was achieved. The described method appears to be suitable for isolation of alpha-lactalbumin in a form adequate for milk formula engineering.

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