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Localization and determination of infarct size by Gd-Mesoporphyrin enhanced MRI in dogs
P Herijgers1, S K Laycock, Y Ni
1Center for Experimental Surgery and Anaesthesiology, K.U. Leuven, Belgium.
Background:
Accurate localization and sizing of a myocardial infarction are necessary for clinical decision making and even more in research. Gd-Mesoporphyrin enhanced magnetic resonance imaging (MRI) was recently shown to specifically delineate necrosis in liver tumors, renal and muscle necrosis and myocardial infarction in rats. In this study, we investigated this technique's potential to accurately delineate myocardial infarction in a larger animal species, the dog.
Methods:
Myocardial infarction was induced in 8 dogs by ligation of the left anterior descending coronary artery, 4 of which were reperfused after 3 hr Gd-Mesoporphyrin (0.05 mmol/kg) was injected intravenously 210 min after the onset of ischemia (n = 6) or after 24 hr in 2 dogs with non-reperfused infarctions. MRI was performed 10 hr after administration of Gd-Mesoporphyrin. In vivo MRI consisted of EKG-triggered, respiratory gated T1-weighted spin echo and segmented turboFLASH long and short axis measurements. Post-mortem, a spin echo short axis measurement was repeated. Infarct size was determined planimetrically by TTC staining of left ventricular slices.
Results:
In all instances, there was a very close qualitative agreement between the MRI and TTC defined myocardial infarction. Quantitatively, the linear regression from post-mortem MRI to TTC determined infarct size yielded a result very close to the line of identity (regression coefficient: 0.980 +/- 0.026, p < 0.000001, adjusted R2 = 0.964).
Conclusion:
We conclude that Gd-Mesoporphyrin enhanced MRI is a promising tool for the accurate delineation of myocardial infarction.
Insights
Gadolinium-Mesoporphyrin enhanced magnetic resonance imaging (MRI) accurately delineates myocardial infarction in dogs. This technique shows high correlation with traditional staining methods for precise infarct sizing.
Area of Science:
- Cardiovascular Imaging
- Medical Diagnostics
- Animal Research Models
Background:
- Accurate myocardial infarction (MI) localization and sizing are crucial for clinical decisions and research.
- Gadolinium-Mesoporphyrin (Gd-MP) enhanced MRI has shown promise in delineating necrosis in various organs.
- This study evaluates Gd-MP enhanced MRI for myocardial infarction delineation in a canine model.
Purpose of the Study:
- To assess the efficacy of Gd-Mesoporphyrin enhanced MRI in accurately delineating myocardial infarction in dogs.
- To compare MRI-based infarct size with results from traditional histopathological staining.
Main Methods:
- Myocardial infarction was induced in 8 dogs; some were reperfused.
- Gd-Mesoporphyrin was administered intravenously at specific time points post-infarction.
- In vivo and post-mortem MRI scans were performed, followed by triphenyltetrazolium chloride (TTC) staining for infarct size determination.
Main Results:
- Qualitative agreement between MRI and TTC staining for myocardial infarction was very close.
- Quantitative analysis showed a strong correlation (R² = 0.964) between post-mortem MRI and TTC determined infarct size, close to the line of identity.
Conclusions:
- Gd-Mesoporphyrin enhanced MRI is a valuable tool for precise myocardial infarction delineation.
- The technique demonstrates high accuracy in sizing infarcts in a large animal model.
- This method holds potential for future research and clinical applications in cardiology.