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Ultrasonic separations in analytical biotechnology

W T Coakley1

  • 1School of Pure and Applied Biology, University of Wales, Cardiff, UK. Coakley@Cardiff.ac.uk

Trends in Biotechnology
|January 7, 1998
PubMed
Summary

Ultrasonic standing waves concentrate cells for easy removal from liquids. This technology enables efficient cell filtration and enhances analytical assay performance in various systems.

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Area of Science:

  • Biotechnology
  • Biophysics

Background:

  • Cell concentration and separation are crucial in biological and chemical processes.
  • Traditional methods for cell manipulation can be inefficient or complex.

Purpose of the Study:

  • To investigate the use of megahertz-frequency ultrasonic standing waves for cell manipulation.
  • To evaluate the effectiveness of ultrasonic filters in cell separation and their application in hybridoma culture.
  • To explore the impact of ultrasonic standing waves on analytical assays.

Main Methods:

  • Utilizing noncavitating ultrasonic standing waves to create cell-free regions.
  • Developing and testing an ultrasonic filter for perfusion hybridoma culture.
  • Assessing the performance of ultrasonic manipulation in aqueous biphasic separations and particle-agglutination assays.
  • Comparing ultrasonic manipulation with immunomagnetic and dielectrophoretic separation techniques.

Main Results:

  • Cells were concentrated at submillimetre distances, forming clumps easily removed from suspension.
  • An ultrasonic filter for perfusion hybridoma culture was successfully developed.
  • Significant increases in the rates of aqueous biphasic separations were observed.
  • Enhanced rates and sensitivities were noted in analytical immunocoated particle-agglutination assays conducted in standing waves.

Conclusions:

  • Ultrasonic standing waves offer an effective method for cell concentration and removal from suspension.
  • The developed ultrasonic filter shows promise for cell separation in perfusion hybridoma culture.
  • Ultrasonic manipulation can significantly improve the efficiency and sensitivity of various biological separations and analytical assays.

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