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Related Experiment Videos

Modulation of T cell-endothelial adhesion by astrocyte conditioned medium

J Joseph1, F D Lublin, R L Knobler

  • 1Department of Neurology, Jefferson Medical College, Philadelphia, Pennsylvania, USA. josephj@allegheny.edu

Glia
|January 7, 1998
PubMed
Summary

Astrocyte factors enhance T cell adhesion to the blood-brain barrier endothelium. This finding is crucial for understanding inflammatory cell entry into the central nervous system in diseases like multiple sclerosis.

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Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Astrocytes are key to maintaining the blood-brain barrier (BBB).
  • Astrocyte-derived factors influence endothelial cell properties and inflammatory cell entry into the brain.
  • Understanding lymphocyte migration through the BBB is vital for central nervous system (CNS) diseases like multiple sclerosis and HIV-induced dementia.

Purpose of the Study:

  • To investigate the role of astrocyte-derived factors in regulating inflammatory cell interactions with the BBB endothelium.
  • To determine how astrocyte conditioned medium affects T cell adhesion to endothelial cells.

Main Methods:

  • Endothelial cells (brain and hepatic) were treated with astrocyte conditioned medium.
  • T cell adhesion to treated endothelial cells was quantified.

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  • Studies utilized fresh activated T cells and T cell hybridomas with specific antigen recognition.

    • Main Results:

    • Astrocyte conditioned medium significantly enhanced T cell adhesion to endothelium in a dose-dependent manner.
    • This enhancement was independent of T cell antigen specificity.
    • Similar results were observed with various T cell types.

    Conclusions:

    • Astrocyte-derived factors promote T cell adhesion to the BBB endothelium.
    • These factors may play a significant role in inflammatory cell infiltration into the CNS.
    • Further research is needed to identify specific astrocyte factors and endothelial adhesion molecules involved.