Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Large-scale temporal gene expression mapping of central nervous system development

X Wen1, S Fuhrman, G S Michaels

  • 1Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.

Proceedings of the National Academy of Sciences of the United States of America
|February 21, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nifedipine maintenance tocolysis and perinatal outcome: an individual participant data meta-analysis.

BJOG : an international journal of obstetrics and gynaecology·2016
Same author

Asymmetry within social groups: division of labour and intergroup competition.

Journal of evolutionary biology·2015
Same author

Pharmacotherapy of Pain in HIV/AIDS.

Journal of back and musculoskeletal rehabilitation·2014
Same author

Preeclampsia and risk of developing subsequent diabetes.

Hypertension in pregnancy·2009
Same author

Are we optimizing gestational diabetes treatment with glyburide? The pharmacologic basis for better clinical practice.

Clinical pharmacology and therapeutics·2009
Same author

Clonidine pharmacokinetics in pregnancy.

Drug metabolism and disposition: the biological fate of chemicals·2009

Researchers mapped gene expression during rat spinal cord development, identifying five distinct developmental "waves" based on signaling genes. This provides a temporal fingerprint for understanding complex genetic networks in development.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genomics

Background:

  • Central nervous system development involves intricate genetic regulation.
  • Understanding temporal gene expression patterns is crucial for deciphering developmental processes.

Purpose of the Study:

  • To create a high-resolution temporal map of mRNA expression for 112 genes during rat cervical spinal cord development.
  • To identify distinct phases and functional relationships of genes during development.

Main Methods:

  • Utilized reverse transcription-coupled PCR to analyze gene expression.
  • Applied distance matrices and cluster analysis to temporal gene expression patterns.
  • Focused on inter- and intracellular signaling genes.

Related Experiment Videos

Main Results:

  • Generated a temporal gene expression "fingerprint" of spinal cord development.
  • Identified five distinct "waves" of gene expression corresponding to developmental phases.
  • Demonstrated that genes from specific functional classes map to particular expression profiles.

Conclusions:

  • The findings suggest functional relationships among genes with parallel expression patterns.
  • The study provides a framework for objectively identifying developmental events in genetic signaling networks.
  • This functional genomics approach may aid in understanding developmental and degenerative disorders.