Analysis of organic and inorganic selenium anions by ion chromatography-inductively coupled plasma atomic emission...

We report analysis of both inorganic and amino acid forms of selenium by ion chromatography with inductively coupled plasma atomic emission spectroscopic detection. Three chromatographic systems are compared; effects of representative sample matrices on the separations are investigated. We are unable to resolve selenate and seleno-cystine using the Dionex AS4A column. Elution of seleno-cystine and seleno-cysteine is strongly suppressed in samples of bacterial cell extract matrix analyzed with the Dionex AS10 column; this interference is not observed with the Dionex AS11 column. Synthetic sea water sample matrix has little effect on analytical results. Quantitation parameters are reported.