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Updated: Aug 16, 2026
Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature
Published on: April 1, 2014
The effect of blood transfusion on susceptibility to bacterial infection in genetically defined mouse models
1University of Cincinnati, College of Medicine, Department of Surgery, and the Shriners Burns Institute, Cincinnati Unit, Ohio 45267-0558, USA.
Background:
Blood transfusions suppress immune function and increase susceptibility to infection, but the effects are not consistent.
Study Design And Methods:
Genetically defined mouse strains with the same or different haplotypes were used as blood transfusion recipients and donors. Transfused animals were subjected to cecal ligation and puncture (CLP) and followed for survival or were injected intravenously with Candida albicans to follow clearance of the Candida from the kidneys.
Results:
BALB/c (H-2d) mice transfused with C3H/HeJ (H-2k) or DBA/2 (H-2d) blood followed by CLP showed significantly lower survival (7 and 10%) than mice transfused with syngeneic blood (61%) or saline controls (56%). Lower survival was also observed in C3H/HeJ (H-2k) mice transfused with BALB/c (H-2d) blood and subjected to CLP (25%) compared with syngeneic transfusion (80%) or saline controls (70%). C57BL/6J (H-2b) mice showed minimal increases in mortality after CLP after transfusion with blood from C3H/HeJ (H-2k) (60% survival), DBA/2 (H-2d) (70% survival), or BALB/c (H-2d) mice (90% survival). When C. albicans was infused intravenously into transfused mice, a similar pattern of altered resistance to infection was found.
Conclusion:
The ability of blood transfusions to increase susceptibility to bacterial infection appears to be dependent on genetic factors unrelated to the major haplotype.
