Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cyclic condensed metaphosphates and linear polyphosphates in brown and red algae

R Niemeyer

    Archives of Microbiology
    |July 1, 1976
    PubMed
    Summary

    This study investigated phosphate metabolism in algae using 32P-orthophosphate labeling. Findings show phosphate incorporation varies with internal reserves, affecting condensed phosphate formation in marine algae.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Condensed phosphates in Lemna minor L. and their relationship to nucleic acids.

    Planta·2014
    Same author

    [Poly- and metaphosphates in higher plants (Lemnaceae)].

    Planta·2014
    Same author

    Subcortical angiopathic encephalopathy in a German kindred suggests an autosomal dominant disorder distinct from CADASIL.

    Acta neuropathologica·2004
    Same author

    Cyclic condensed metaphosphates in plants and the possible correlations between inorganic polyphosphates and other compounds.

    Progress in molecular and subcellular biology·1999
    Same author

    Randomized comparison of G-CSF + GM-CSF vs G-CSF alone for mobilization of peripheral blood stem cells: effects on hematopoietic recovery after high-dose chemotherapy.

    Bone marrow transplantation·1998
    Same author

    Antifungal effects of yeast-derived rhu-GM-CSF in patients receiving high-dose chemotherapy given with or without autologous stem cell transplantation: a retrospective analysis.

    Bone marrow transplantation·1996

    Area of Science:

    • Marine Biology
    • Biochemistry
    • Phycology

    Background:

    • Condensed phosphates, including linear polyphosphates and cyclic metaphosphates, play crucial roles in cellular energy storage and metabolism.
    • Understanding the synthesis and fate of these phosphates is vital for comprehending algal physiology and nutrient cycling.

    Purpose of the Study:

    • To investigate the occurrence and dynamics of linear and cyclic condensed phosphates in various Phaeophyceae and Rhodophyceae species.
    • To elucidate the influence of cellular phosphate reserves on the incorporation and distribution of 32P-orthophosphate.

    Main Methods:

    • Pulse-labeling experiments using 32P-orthophosphate (3-5 hours) were conducted on selected brown and red algae species.
    • Two-dimensional cellulose thin-layer chromatography and MAK-chromatography were employed to separate and analyze phosphate compounds.
    • Direct scanning with a Geiger-Muller counter quantified radioactivity in different phosphate fractions.

    Main Results:

    • All studied algae species incorporated 32P into various oligopolyphosphates (2-7 residues), cyclic metaphosphates (tri-, tetra-, penta-, hexametaphosphates), and high-molecular-weight condensed phosphates.
    • Trimetaphosphate exhibited significantly higher specific activity among low-molecular-weight condensed inorganic phosphates.
    • Phosphate uptake and incorporation into high-molecular-weight fractions were dependent on internal phosphate pools; low incorporation occurred in nutrient-rich media, while high incorporation into high-molecular-weight fractions was observed after P-depletion.

    Conclusions:

    • Algal phosphate metabolism involves the formation of diverse condensed phosphate forms, with trimetaphosphate being a key low-molecular-weight intermediate.
    • Cellular phosphate storage significantly regulates the incorporation of inorganic phosphate into high-molecular-weight condensed phosphates, including those associated with nucleic acids.

    Related Experiment Videos