Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitochondria transfer into mouse ova by microinjection

C A Pinkert1, M H Irwin, L W Johnson

  • 1Department of Comparative Medicine, University of Alabama at Birmingham 35294-0019, USA.

Transgenic Research
|January 10, 1998
PubMed
Summary

Researchers successfully transferred mitochondria between mouse species, demonstrating a new method for studying mitochondrial function and potential treatments for metabolic diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Anabolic Steroids on Lipoprotein Profiles of Female Weight Lifters.

The Physician and sportsmedicine·2016
Same author

Effects of Intertidal Harvest Practices on Levels of Vibrio parahaemolyticus and Vibrio vulnificus Bacteria in Oysters.

Applied and environmental microbiology·2016
Same author

Mitochondrial DNA sequence and phylogenetic evaluation of geographically disparate Sus scrofa breeds.

Animal biotechnology·2014
Same author

Together.

The Journal of the Louisiana State Medical Society : official organ of the Louisiana State Medical Society·2012
Same author

Navy hospitals underground.

Modern hospital·2010
Same author

Xenomitochondrial mice: investigation into mitochondrial compensatory mechanisms.

Mitochondrion·2010

Area of Science:

  • Mitochondrial biology
  • Reproductive biology
  • Genetics

Background:

  • Mitochondria play crucial roles in cellular energy production and are implicated in various metabolic diseases.
  • Developing methods for interspecific mitochondrial transfer is essential for studying mitochondrial inheritance and function.
  • Investigating mitochondrial dynamics requires reliable techniques for isolating and transferring mitochondria between species.

Observation:

  • Mitochondria were isolated from Mus spretus liver cells.
  • These isolated mitochondria were microinjected into fertilized M. musculus domesticus ova.
  • Electron microscopy confirmed the integrity of isolated mitochondria with minimal contamination.

Findings:

  • Species-specific PCR confirmed successful transfer of foreign mitochondria into recipient zygotes.

Related Experiment Videos

  • A significant percentage of injected zygotes developed into blastocysts.
  • All developed blastocysts contained detectable levels of the transferred foreign mitochondria.

    • Implications:

    • This study establishes a novel model system for investigating mitochondrial dynamics in vivo.
    • The developed method could pave the way for therapeutic strategies targeting mitochondrial dysfunction in human diseases.
    • Further research can explore the long-term effects and applications of interspecific mitochondrial transfer.