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Related Experiment Videos

Cortisol increases transfection efficiency of cells

A G Bernasconi1, A G Rebuffat, E Lovati

  • 1Dept. Internal Medicine, University Hospital Bern, Switzerland.

FEBS Letters
|January 13, 1998
PubMed
Summary

Glucocorticoids like cortisol significantly enhance DNA transfection efficiency by increasing reporter gene uptake and expression. This effect is mediated by activated glucocorticoid receptors promoting DNA nuclear translocation.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Transfection is a key technique for introducing nucleic acids into cells.
  • Glucocorticoids are steroid hormones with diverse cellular functions.
  • The role of glucocorticoids in modulating DNA delivery into cells remains incompletely understood.

Purpose of the Study:

  • To investigate the effect of glucocorticoids on DNA transfection efficiency.
  • To elucidate the mechanism by which glucocorticoids influence DNA uptake and expression.

Main Methods:

  • Cells were transfected with reporter gene constructs in the presence or absence of glucocorticoids.
  • Reporter gene expression levels were quantified to assess transfection efficiency.
  • Dose-response experiments and antagonist studies were performed.

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Main Results:

  • Physiological concentrations of 11beta-hydroxy glucocorticoids, such as cortisol, significantly enhanced reporter gene expression.
  • Cortisol treatment resulted in a two-fold increase in cellular uptake and expression of the reporter gene.
  • The enhancing effect was specific to active glucocorticoids, dose-dependent, and blocked by the glucocorticoid antagonist RU486.

Conclusions:

  • Activated cytoplasmic glucocorticoid receptors play a crucial role in facilitating DNA transfection.
  • Glucocorticoid receptor activation appears to enhance the nuclear translocation of incoming transfected DNA.
  • This finding offers a novel strategy for improving gene delivery in research and therapeutic applications.