Related Experiment Videos
A novel, negative selectable marker for gene disruption in Dictyostelium
A Morrison1, R Marschalek, T Dingermann
1The Imperial Cancer Research Fund, Clare Hall Laboratory, South Mimms, Herts, UK.
Gene
|January 14, 1998
Abstract:
The expression of an ochre suppressor mutant of the GluII(UUA) tRNA appears to be lethal to Dictyostelium, and offers a novel 'positive negative' strategy to select for targeted gene disruption by homologous recombination. Inclusion of the suppressor tRNA gene decreases the overall transformation frequency by approximately 20-fold. This increases the proportion of targeted gene disruptions to over 90%.