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Molecular characterization of a new human minisatellite that is able to form single-stranded loops in vitro and is
F Boán1, A I González, J M Rodríguez
1Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Santiago, Galicia, Spain.
FEBS Letters
|January 15, 1998
Abstract:
We report the isolation of a new low polymorphic GC-rich human minisatellite locus (MsH42) that contains different recombination motifs and is homologous to sequences involved in immunoglobulin class-switching. Furthermore, we show that MsH42 undergoes slipped-strand mispairing during PCR indicating its ability to generate single-stranded loops. Specific DNA-protein complexes were detected in band-shifting experiments using nuclear extracts from mouse testes and human NC-37 cells. The possible implications of this minisatellite in recombination events is discussed.