Related Experiment Video
Updated: Aug 11, 2026
MRI and PET in Mouse Models of Myocardial Infarction
Published on: December 19, 2013
Technetium-99m MIBI to assess coronary collateral flow during acute myocardial infarction in two closed-chest animal
T F Christian1, M K O'Connor, R S Schwartz
1Department of Nuclear Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA.
Unlabelled:
Collateral flow is an independent determinant of infarct size in both animal and clinical studies of myocardial infarction. The purpose of this study was to quantitatively evaluate, in a closed-chest animal model, a noninvasive method of measuring coronary collateral flow over a wide spectrum of collateral flow rates from a tracer that can be injected during occlusion but measured after reperfusion.
Methods:
Fourteen animals underwent 40 min of coronary occlusion using a closed-chest technique. Two closed-chest models representing different rates of collateral flow were used: canine and porcine. Coronary blood flow was measured by radiolabeled microspheres. Collateral blood within the risk zone was estimated from the severity of 99mTc-sestamibi tomographic perfusion defect.
Results:
Collateral blood flow was significantly higher in the canine model than it was in the porcine model. There was close agreement (r = 0.90) between absolute collateral flow by microspheres and the severity of the tomographic perfusion defect.
Conclusion:
These results suggest that an accurate noninvasive estimate of collateral blood flow can be provided by an intravenous injection of 99mTc-sestamibi.
