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Development of a murine model of Helicobacter pylori infection
L B Lachman1, B Ozpolat, X M Rao
1Department of Cell Biology, University of Texas M. D. Anderson Cancer, Houston 77030, USA. Lachman@odin.mdacc.tmc.edu
Background:
A murine model for Helicobacter pylori infection could facilitate vaccine development. This study was designed to determine the effect of various conditions of dose, frequency of administration, and fasting on H. pylori infection of mice.
Materials And Methods:
Balb/c and C3H/HeN mice were inoculated orogastrically with clinical isolates of H. pylori grown in liquid culture. At 2-week intervals, the stomachs were removed for secondary culture on horse blood agar and for histological analysis. H. pylori from secondary cultures or homogenized stomach tissue from infected mice was inoculated a second time in naïve animals.
Results:
H. pylori was cultured with high frequency only from the stomachs of C3H/HeN mice. Fasting the mice and increasing the number of organisms inoculated did not increase the rate of infection. Histological analysis detected no inflammation, but mucus depletion and erosion were present in the stomachs of C3H/HeN mice. H. pylori organisms were not observed. Secondary cultures of H. pylori or homogenized infected stomach tissue did not cause infection when inoculated in naïve mice.
Conclusions:
Clinical isolates of H. pylori transiently infect C3H/HeN mice. This murine model is suitable for testing oral vaccines. Effective vaccination against H. pylori could prevent transient infection and reduce subsequent gastritis.
Insights
Helicobacter pylori transiently infects C3H/HeN mice, establishing a model for oral vaccine testing. This research explored dose, frequency, and fasting effects on infection rates in mice.
Area of Science:
- Microbiology
- Immunology
- Veterinary Science
Background:
- Developing a murine model for Helicobacter pylori infection is crucial for advancing vaccine development.
- This study investigated factors influencing H. pylori infection in mice to optimize a potential model.
Purpose of the Study:
- To evaluate the impact of varying inoculation doses, administration frequencies, and fasting periods on H. pylori infection rates in mice.
- To assess the suitability of a murine model for H. pylori infection in the context of vaccine development.
Main Methods:
- Balb/c and C3H/HeN mice were orogastrically inoculated with clinical H. pylori isolates.
- Stomach tissue was analyzed via secondary culture and histology at 2-week intervals.
- Infectivity was tested by re-inoculating naive mice with cultured H. pylori or homogenized stomach tissue.
Main Results:
- H. pylori was consistently cultured from C3H/HeN mice, but not Balb/c mice.
- Infection rates were not significantly increased by fasting or higher inoculation doses.
- Histological examination revealed mucus depletion and erosion, but no inflammation or observable H. pylori organisms.
Conclusions:
- The C3H/HeN mouse model supports transient H. pylori infection, making it suitable for oral vaccine efficacy trials.
- This model can aid in developing vaccines to prevent H. pylori infection and mitigate associated gastritis.