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A circular plasmid from the yeast Torulaspora delbrueckii
J Blaisonneau1, F Sor, G Cheret
1Institut Curie Section de Recherche, UMR 216, Centre Universitaire Paris XI, Orsay, France.
Plasmid
|January 1, 1997
Summary
A novel plasmid, pTD1, was discovered in Torulaspora delbrueckii yeast. This finding expands the known 2-micron plasmid family and establishes a new host-vector system for this yeast species.
Area of Science:
- Microbiology
- Molecular Biology
- Yeast Genetics
Background:
- The 2-micron plasmid family is a well-characterized group of circular DNA molecules found in certain yeast species.
- Previous studies indicated this plasmid family was restricted to a few closely related genera.
Purpose of the Study:
- To identify and characterize new members of the 2-micron plasmid family.
- To develop a functional host-vector system for Torulaspora delbrueckii.
Main Methods:
- Nucleotide sequencing of the isolated plasmid pTD1.
- Replication studies in Saccharomyces cerevisiae and T. delbrueckii to identify the ARS region.
- Construction and testing of pTD1 derivatives in auxotrophic mutant hosts.
Main Results:
- A new plasmid, pTD1, was identified in Torulaspora delbrueckii, containing inverted repeats and open reading frames, including a FLP recombinase homologue.
- An autonomously replicating sequence (ARS) region was located near an inverted repeat.
- An efficient host-vector system for T. delbrueckii was established using pTD1 derivatives.
Conclusions:
- The 2-micron plasmid family has been expanded to include Torulaspora delbrueckii.
- The discovery of pTD1 and the development of a host-vector system facilitate genetic manipulation of T. delbrueckii.
- The 2-micron plasmid family's host range is confirmed to be limited to specific yeast genera.