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Related Experiment Videos

A new repetitive sequence uniquely present in the decay-accelerating factor genes

M Nonaka1, O Takenaka, N Okada

  • 1Department of Molecular Biology, Nagoya City University School of Medicine, Mizuho-ku, Nagoya 467, Japan.

Immunogenetics
|March 7, 1998
PubMed
Summary

The decay-accelerating factor (DAF, CD55) gene contains repetitive sequences in its exon and intron regions, suggesting ancient origins. These repetitive structures may have functional roles, potentially including translation of intron sequences.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Immunology

Background:

  • The decay-accelerating factor (DAF, CD55) is crucial for protecting cells from complement-mediated damage.
  • Previous research identified variable-length serine/threonine-rich (S/T)-abc regions in guinea pig DAF due to differential exon repeat usage.
  • Alternative splicing in the DAF S/T region is known in some species, leading to protein diversity.

Purpose of the Study:

  • To investigate the repetitive structures within the DAF gene, specifically focusing on the seventh exon and intron.
  • To determine if these repetitive sequences are conserved across species and their evolutionary origins.
  • To explore the potential functional implications of these repetitive sequences, including possible intron translation.

Main Methods:

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  • Comparative genomic analysis of DAF genes from various species, including humans, primates, and mice.
  • Sequence analysis of the seventh exon and intron of the DAF gene to identify repetitive elements.
  • In silico translation of intron sequences to assess for the presence of stop codons in relevant reading frames.

    • Main Results:

    • The seventh intron of the guinea pig DAF gene consists of 18 tandem repeats homologous to the S/T-abc exon repeats.
    • Similar repetitive structures spanning exon and intron regions were found in DAF genes of human, primates, and mice, indicating ancient evolutionary origins.
    • Intron sequences, when translated in the same frame as adjacent exons, generally lack stop codons, suggesting potential for translation.

    Conclusions:

    • The repetitive sequences in DAF exons and introns arose before species diversification, highlighting their conserved nature.
    • The conserved, non-stop codon containing intron sequences suggest a potential, yet unconfirmed, functional role, possibly involving translation.
    • Further research is needed to confirm the expression and function of these translated intron sequences in DAF.