Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Amidative peptide processing and vascular function

C D Oldham¹, C Li, J Feng

¹School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30332, USA.

The American Journal of Physiology

|January 22, 1998

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Changes in Chromatographic Profile of Anthocyanins of Red Onion During Extraction.

Journal of food protection·2019

Same author

Acylated Anthocyanins in Red Onions.

Journal of food protection·2019

Same author

A low concentration of genistein induces estrogen receptor-alpha and insulin-like growth factor-I receptor interactions and proliferation in uterine leiomyoma cells.

Human reproduction (Oxford, England)·2008

Same author

Stimulatory and inhibitory effects of genistein on human uterine leiomyoma cell proliferation are influenced by the concentration.

Human reproduction (Oxford, England)·2007

Same author

Influences of parity and level of feed intake on reproductive response to insulin administration after weaning in sows.

Journal of animal science·2002

Same author

Estrogen receptor-alpha knockout mice exhibit resistance to the developmental effects of neonatal diethylstilbestrol exposure on the female reproductive tract.

Developmental biology·2002

Same journal

Blood coagulation in fish.

The American journal of physiology·2011

Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010

Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010

Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010

Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010

Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010

See all related articles

The precursor to Substance P (SP), SP-Gly, relaxes blood vessels by activating nitric oxide synthase (NOS). This process requires amidative processing, highlighting its role in basal endothelial cell NOS activity and vasodilation.

Area of Science:

Cardiovascular Physiology
Endocrinology
Biochemistry

Background:

Substance P (SP), a sensory nerve peptide, influences cardiovascular function.
Exogenous SP activates nitric oxide synthase (NOS) in endothelial cells, impacting vasodilation.

Purpose of the Study:

To investigate the role of SP-Gly, the precursor to SP, in vasodilation.
To determine if amidative processing is essential for SP-Gly's vasodilatory effects and NOS activation.

Main Methods:

Vascular relaxation assays using rat aortic strips.
Treatment with 4-phenyl-3-butenoic acid (PBA), an amidating enzyme inhibitor.
Measurement of endothelial cell nitric oxide synthase (NOS) activity.
Affinity chromatography and Western blot analysis for amidating enzymes.

Related Experiment Videos

Main Results:

SP-Gly induced vasodilation comparable to SP.
PBA inhibited SP-Gly's vasodilatory effect but not SP's, indicating a requirement for amidative processing.
PBA decreased basal NOS activity in endothelial cells without causing cell death or directly inhibiting NOS.
Endothelial cells possess both bifunctional and monofunctional amidating enzymes.

Conclusions:

Amidative processing of SP-Gly is crucial for its vasodilatory action.
This processing pathway is linked to NOS activation in endothelial cells.
Products of amidative processing contribute to the basal activation of NOS in endothelial cells, influencing cardiovascular function.