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Electron microscopic observation of delta-opioid receptor-1 in the rat area postrema
1Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
Abstract:
The ultrastructural localization of delta-1-opioid-receptor in the rat area postrema was quantitatively studied by pre-embedding avidin-biotin-peroxidase-complex technique. Most of the immunoreactive profiles (67.4%) observed in the present study were axon terminals, whereas the immunopositive dendrites were less (28.3%). Within the axon terminals, the immunoreactivity was found stronger in the dense-cored vesicles than in the small, clear, and round vesicles. Almost 2/3 of the DOR-1 immunoreactive axon terminals had DAB reacted dense-cored vesicles. About half of the immunopositive axon terminals were found to make synapse to dendrites. The dendrites postsynaptic to DOR-1 immunoreactive axon terminals were identified as DOR-1 immunoreactive or not, mainly according to the immunoreactive appearance of the postsynaptic membrane. About half of the DOR-1 immunoreactive dendrites were observed to receive synapse: most of them have their immunoreactivity results at the postsynaptic membranes.