Related Experiment Videos
Expression of genes encoding membrane-bound hydrogenase in Pseudomonas hydrogenovora under autotrophic condition is
T Ohtsuki1, M Shimosaka, M Okazaki
1Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Abstract:
Expression of a membrane-bound hydrogenase of the hydrogen-utilizing bacterium Pseudomonas hydrogenovora was induced specifically in autotrophic condition. Dot blot and primer extension analysis showed that the transcription of the hydrogenase gene started from the region upstream of a hydrogenase structural gene, hupS, which contained three putative sigma54-type promoter sequences (Phup1, Phup2, and Phup3). The lacZ-fusion analysis of the hupS-upstream region combined with site-directed mutagenesis showed that Phup1 and Phup3 would be essential for a transcriptional initiation. It was also found that the region upstream of Phup sequences was concerned with regulation of transcription.