The A and B forms of plastid DNA-dependent RNA polymerase from mustard (Sinapis alba L.) transcribe the same genes in...

Two RNA polymerases, termed A (cp-pol A) and B (cp-pol B), are known to be present in mustard plastids. In vitro, the two enzymes have different requirements for DNA binding, but both bind to, and transcribe from, the same set of chloroplast promoters. The B enzyme is sensitive to rifampicin (Rif), whereas the A enzyme is not. When seedlings were grown in the presence of Rif, RNA pool sizes of the photosynthesis-related plastid genes rbcL and psbA were smaller than in untreated controls, whereas transcripts of the non-photosynthetic genes rps16, trnG, rrn and rpoB remained virtually unaffected by the drug. The Rif inhibition patterns of rbcL and psbA transcripts reflect the relative abundance of the A and B enzymes at different stages and light/dark conditions. These genes can thus be transcribed by either of the two enzymes in vivo, whereas the non-photosynthetic genes are transcribed mostly or exclusively by the A enzyme, or by another Rif-resistant plastid polymerase. Among several nuclear gene transcripts that were tested for Rif inhibition, only those of the RbcS gene family for the plastid-bound small subunit of Rubisco revealed a decrease in pool size, which may imply that mechanisms exist that serve to coordinate patterns of gene expression in the different cellular compartments.