[Biotransformation ability of the liver in ischemic reperfusion injury]

The aim of the study was to estimate the activity of enzymes connected with xenobiotics biotransformation in early reperfusion period after 90 minutes ischemia of rat liver and to state the correlation of obtained results with values of standard liver tests. Full, global liver ischemia was performed using Isozaka model with temporary portocaval anastomosis. The activity of alanin and aspargine aminotransferase and lactate dehydrogenase in blood serum, and P-450 and b5 cytochromes with related to them reductases in microsomal fractions of liver homogenates were estimated in 24th hour of reperfusion. Considerable decrease of the activity of mixed function oxidase system, not correlating with the results of standard liver tests was observed.