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Related Experiment Videos

Readily releasable pool size changes associated with long term depression

Y Goda1, C F Stevens

  • 1Molecular Neurobiology Laboratory and Howard Hughes Medical Institute, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 14, 1998
PubMed
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Long-term depression (LTD) in hippocampal neurons reduces the readily releasable pool size. This depression is synapse-specific on the target neuron but partially affects other synapses from the same presynaptic neuron.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Synaptic Plasticity

Background:

  • Autaptic connections in cultured hippocampal neurons are crucial for understanding synaptic function.
  • Long-term depression (LTD) is a key mechanism for synaptic plasticity.
  • The readily releasable pool (RRP) of synaptic vesicles is fundamental to neurotransmission.

Purpose of the Study:

  • To quantify the readily releasable pool size in hippocampal neurons before and after LTD induction.
  • To investigate the synapse specificity of LTD in autaptically and reciprocally interconnected neurons.

Main Methods:

  • Primary cultures of hippocampal neurons were used.
  • Stimulation protocols were employed to induce long-term depression (LTD).
  • Electrophysiological recordings monitored synaptic transmission and readily releasable pool size.

Related Experiment Videos

Main Results:

  • LTD induction led to a decrease in the readily releasable pool size, proportional to synaptic current depression.
  • Synaptic strength changes were confined to the active synapses on the target neuron during LTD induction.
  • Presynaptically, synapses active during LTD induction showed partial depression, even if not on the target neuron.

Conclusions:

  • LTD in this autaptic hippocampal neuron model involves a reduction in the readily releasable pool.
  • The depression of synaptic strength is highly specific to the stimulated synapses on the postsynaptic neuron.
  • Presynaptic expression of LTD is not absolute, with partial depression observed at non-target synapses.