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M phase phosphoprotein 10 is a human U3 small nucleolar ribonucleoprotein component
J M Westendorf1, K N Konstantinov, S Wormsley
1Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Molecular Biology of the Cell
|April 4, 1998
Summary
We identified a novel M phase phosphoprotein, MPP10, which localizes to the nucleolus and associates with U3 small nucleolar RNA (snoRNA), suggesting a role in rRNA processing.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- M phase phosphoproteins (MPPs) are crucial for cell division.
- MPP10 is a newly identified protein with an unknown function.
Purpose of the Study:
- To investigate the nucleolar function of MPP10.
- To characterize MPP10's localization and interactions within the cell.
Main Methods:
- Cell fractionation
- Immunofluorescence microscopy
- Actinomycin D treatment
- Immunoprecipitation
- RNA isolation
Main Results:
- MPP10 is predominantly found in isolated nucleoli.
- MPP10 colocalizes with fibrillarin in interphase nucleoli and on chromosomes during M phase.
- MPP10 localizes to the fibrillar domain of nucleoli, involved in rRNA processing.
- MPP10 interacts with U3 small nucleolar RNA (snoRNA).
Conclusions:
- MPP10 plays a role in nucleolar function, likely in rRNA processing.
- MPP10 is a component of the human U3 small nucleolar ribonucleoprotein complex.