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Lysogeny in Streptococcus bovis

W G Iverson, N F Millis

    Canadian Journal of Microbiology
    |June 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Mitomycin C induction revealed bacteriophage-like particles in eleven Streptococcus bovis strains. Lysogenic Streptococcus bovis strains are unlikely to be a significant source of bacteriophages in the rumen environment.

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    Succession of Streptococcus bovis strains with differing bacteriophage sensitivities in the rumens of two fistulated sheep.

    Applied and environmental microbiology·1977

    Area of Science:

    • Microbiology
    • Bacteriology
    • Virology

    Background:

    • Streptococcus bovis is a bacterium found in the gastrointestinal tract of ruminants.
    • Bacteriophages are viruses that infect bacteria and play a role in microbial ecology.
    • The lysogenic potential of Streptococcus bovis strains and their contribution to bacteriophage populations are not well understood.

    Purpose of the Study:

    • To investigate the lysogenic potential of Streptococcus bovis strains.
    • To characterize bacteriophage-like particles produced by inducible Streptococcus bovis strains.
    • To assess the prevalence of lysogenic Streptococcus bovis in different ecological niches, particularly the rumen.

    Main Methods:

    • Forty-eight strains of Streptococcus bovis were cultured and subjected to mitomycin C induction.

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  • Bacterial lysates were examined for the presence of bacteriophage-like particles using electron microscopy.
  • Lysates were tested for antimicrobial activity against indicator strains to detect viable bacteriophages.

    • Main Results:

    • Eleven out of 48 Streptococcus bovis strains were inducible by mitomycin C.
    • Bacteriophage-like particles were detected in the lysates of all 11 inducible strains.
    • Viable bacteriophages were isolated from six of the induced lysates, with one lysate containing polyhead-like structures.
    • Only one of 23 rumen-derived Streptococcus bovis strains was found to be lysogenic.

    Conclusions:

    • Mitomycin C induction is effective in identifying lysogenic Streptococcus bovis strains.
    • Streptococcus bovis can harbor and produce bacteriophages.
    • Lysogenic Streptococcus bovis strains appear to be uncommon in the rumen, suggesting they are not a major source of bacteriophages in this environment.