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Studies towards a disposable screen-printed amperometric biosensor for progesterone

J P Hart1, R M Pemberton, R Luxton

  • 1Faculty of Applied Sciences, University of the West of England, Bristol, UK.

Biosensors & Bioelectronics
|January 1, 1997
PubMed
Summary

This study developed a disposable biosensor for progesterone analysis using a screen-printed carbon electrode and an anti-progesterone antibody. The biosensor achieved a detection limit of 1 x 10(-9) mol/dm3, showing potential for biological fluid analysis.

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Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Immunochemistry

Background:

  • Development of sensitive and selective biosensors is crucial for accurate hormone level monitoring.
  • Screen-printed carbon electrodes (SPCEs) offer a cost-effective and disposable platform for electrochemical biosensors.
  • Monoclonal antibodies provide high specificity for target analyte recognition.

Purpose of the Study:

  • To develop and validate a disposable amperometric progesterone biosensor.
  • To optimize the immobilization of anti-progesterone antibody on SPCE for enhanced performance.
  • To evaluate the biosensor's performance in a competitive immunoassay format.

Main Methods:

  • Immobilization of monoclonal sheep anti-progesterone antibody onto SPCE via rabbit IgG.

Related Experiment Videos

  • Competitive assay utilizing alkaline phosphatase-labelled progesterone and phenyl phosphate substrate.
  • Electrochemical detection using cyclic voltammetry, amperometry, and chronoamperometry to measure phenol production.

    • Main Results:

    • Optimized antibody loading conditions were established for the SPCE transducer.
    • Phenol, a product of phenyl phosphate hydrolysis, exhibited favorable electrochemical characteristics.
    • The chronoamperometric response showed a progesterone-dependent range over three orders of magnitude.
    • A detection limit of approximately 1 x 10(-9) mol/dm3 for progesterone was achieved.

    Conclusions:

    • The developed disposable amperometric progesterone biosensor is sensitive and selective.
    • The biosensor demonstrates potential for the quantitative analysis of progesterone in biological fluids.
    • This technology offers a promising tool for clinical diagnostics and research applications.