[Structural rearrangement in leukocyte membranes under the effect of quercetin and linoleic acid hydroxamate]

The character of structural rearrangements in leukocyte membranes affected by 5-lipoxygenase inhibitors: quercetin and linoleic acid hydroxamate, has been investigated. Quercetin has been shown to induce the translocation of tryptophanyls and tyrosyls from membrane protein inner regions to their surface. Linoleic acid hydroxamate produces the analogous transition of tyrosine residues only. Quercetin brings out disturbances of surface membrane proteins as was registered by ANS fluorescent parameters. It is likely able to arise from the increase of protein hydration. The linoleic acid hydroxamate elevates the quantity of ANS binding sites on the membrane surfaces without any change in their structural features. This effect is likely induced by the surface charge modification of the leukocyte membranes. The linoleic acid hydroxamate increases the level of protein descent into the lipid matrix and decreases the polarity and microviscosity of hydrophobic regions of the latter.