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Published on: February 2, 2009
Specific monoclonal antibody raised against the p33ING1 tumor suppressor
I Garkavtsev1, D Boland, J Mai
1Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Abstract:
An IgG1 mouse monoclonal antibody (CAb1) was produced against human recombinant p33ING1. The antibody is able to recognize native and denatured antigen in ELISA and Western blot protocols, respectively. CAb1 can be used to specifically detect the p33ING1 protein in dot blot and Western immunoblot protocols of both human and mouse cell lysates. In addition, this antibody is also useful for cellular localization of native and ectopically overexpressed p33ING1 protein by indirect immunofluorescence.
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