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Updated: Aug 11, 2026
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Published on: June 26, 2018
Cytokine production in cell culture by peripheral blood mononuclear cells from immunocompetent hosts
R K Katial1, D Sachanandani, C Pinney
1Department of Allergy and Immunology, Walter Reed Army Medical Center, Washington, DC 20307, USA. RKatial@POL.NET
Abstract:
The production of interleukin 2 (IL-2) gamma interferon, IL-4, tumor necrosis factor alpha (TNF-alpha), TNF-beta, IL-5, and IL-10 in vitro by peripheral blood mononuclear cells cultured from healthy immunocompetent subjects after mitogen stimulation was determined. The mitogens used were concanavalin A, phytohemagglutinin, pokeweed mitogen, and Staphylococcus aureus Cowen. The results obtained provide a normal range for the production of these cytokines under specified conditions in vitro.
