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Pre-, on- and post-column derivatization in capillary electrophoresis
H A Bardelmeijer1, J C Waterval, H Lingeman
1Department of Pharmaceutical Analysis, Utrecht University, The Netherlands.
Electrophoresis
|February 10, 1998
Summary
This survey overviews derivatization reagents and procedures for capillary electrophoresis (CE). It details methods for various functional groups, emphasizing amino group labeling for enhanced analysis.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrophoresis (CE) is a powerful analytical technique.
- Derivatization enhances analyte detection and separation in CE.
- Comparing CE derivatization with liquid chromatography is crucial for method selection.
Purpose of the Study:
- To provide a comprehensive overview of derivatization reagents and procedures in CE.
- To discuss the advantages and disadvantages of pre-, post-, and on-column derivatization modes.
- To highlight reagents for labeling various functional groups, with a focus on amino groups.
Main Methods:
- Literature review of derivatization reagents and procedures for CE.
- Categorization of reagents based on functional groups (amino, aldehyde, keto, carboxyl, hydroxyl, sulfhydryl).
- Compilation of data in tables detailing detection modes, analytes, sensitivity, and sample types.
Main Results:
- Detailed discussion of reagents for amino group derivatization in CE.
- Inclusion of information on labeling for aldehyde, keto, carboxyl, hydroxyl, and sulfhydryl groups.
- Tables summarizing key parameters for various derivatization methods.
Conclusions:
- Derivatization is essential for optimizing CE analysis of various compounds.
- The choice of derivatization mode and reagent depends on the analyte and desired outcome.
- This survey serves as a valuable resource for selecting appropriate CE derivatization strategies.