Some aspects of immunodiagnosis in amebiasis

Antigen from axenically and with crithidia monoxenically grown amebae are compared in complement fixation test and indirect hemagglutination test. While similar reactivity was observed in indirect hemagglutination monoxenic antigen yielded extremely higher titers in complement fixation tests. Transient latex agglutinations of severely ill malaria tropica patients proved to be spontaneous in nature. It is recommended that extra latex is added to the test kits to provide for a check of positive sera for spontaneous agglutination. Glutaraldehyde treated and sensitized sheep red cells were stored under different conditions and their reactivity assessed over various periods of time. No loss of reactivity was found to occur under either condition after the respective times of observation, the longest of which lasted 12 months.