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Paracoccidioides cerebriformis Moore, 1935. Mycologic and immunochemical study

C da S Lacaz1, M S Vidal, C N Pereira

  • 1Barnes Hospital, St. Louis, Missouri, USA.

Revista Do Instituto De Medicina Tropical De Sao Paulo
|May 1, 1997
PubMed
Summary

This study re-evaluated Paracoccidioides cerebriformis, finding it lacks key antigens and does not cause experimental lesions. Findings suggest Paracoccidioidomycosis results solely from Paracoccidioides brasiliensis infection.

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Area of Science:

  • Medical Mycology
  • Immunochemistry
  • Infectious Diseases

Background:

  • The fungus Paracoccidioides cerebriformis was described by Moore in 1935.
  • The species has been maintained in culture for 60 years, exhibiting consistent characteristics.
  • Previous classification placed P. cerebriformis within the Paracoccidioides genus.

Purpose of the Study:

  • To mycologically and immunochemically re-evaluate the fungus P. cerebriformis.
  • To determine if P. cerebriformis is a distinct species within the Paracoccidioides genus.
  • To clarify the etiological agent of Paracoccidioidomycosis.

Main Methods:

  • Mycologic culture and characterization.
  • SDS PAGE and Western blotting to detect specific antigens.

Related Experiment Videos

  • Immunoelectrophoresis using anti-gp43 serum.
  • Intratesticular inoculation in guinea pigs to assess pathogenicity.

    • Main Results:

    • The fungus maintained its characteristics over 60 years in culture.
    • No experimental lesions were observed in guinea pigs inoculated with the fungus.
    • The dominant antigen gp43 of Paracoccidioides brasiliensis was not detected.
    • Immunoelectrophoresis did not show the characteristic E arch with anti-gp43 serum.

    Conclusions:

    • The fungus described as P. cerebriformis does not belong to the genus Paracoccidioides.
    • Paracoccidioidomycosis is likely caused by a single species, Paracoccidioides brasiliensis.
    • Further molecular studies are recommended for definitive identification of the fungus.