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Polyvinyl alcohol-glutaraldehyde as solid-phase in ELISA for schistosomiasis
A M Araújo1, G H Barbosa, J R Diniz
1Laboratório de Immunopatologia Keizo Asami, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
Abstract:
Soluble adult Schistosoma mansoni antigen preparation (SWAP) was covalently fixed onto polyvinyl alcohol-glutaraldehyde discs and an enzyme linked-immunosorbent assay (ELISA) was set up. The best conditions for the assay were established and it was found that small amount of antigen such as 1.5 micrograms was required. A comparison between this procedure and the conventional ELISA was proceeded. A reliable method of antigen immobilization was achieved and the low prices of the employed reagents are economically attractive.