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[The nature of the interactions of imipramine and serotonin with human lymphocytes]
S A Lesnichuk1, V Iu Katukov, N V Porodenko
1Department of Biochemistry, Sechenov Moscow Medical Academy, Moscow, Russia.
The work deals with the study of tritium-labeled serotonin (3H-5-hydroxytryptamine, 3H-5-HT) binding with mononuclear leukocytes (lymphocytes) isolated from human peripheral blood. It was shown that 3H-5-HT interacts specifically with the lymphocytes according to the type of reversible, saturated, and temperature-dependent receptor binding. The parameters of binding of the dissociation constant of the ligand-receptor complex and the maximum concentration of the binding centers were determined. It was shown that the tricyclic antidepressant imipramine inhibits the binding of 3H-5-HT with IC50 = 280 nm. The binding of 3H-5-HT with lymphocytes is Na-insensitive but is inhibited by Ca and Mg ions. The interrelation of 3H-5-HT with the lymphocytes differs in nature from the process of reuptake capture of 3H-5-HT by the blood platelets.
The work deals with the study of tritium-labeled serotonin (3H-5-hydroxytryptamine, 3H-5-HT) binding with mononuclear leukocytes (lymphocytes) isolated from human peripheral blood. It was shown that 3H-5-HT interacts specifically with the lymphocytes according to the type of reversible, saturated, and temperature-dependent receptor binding. The parameters of binding of the dissociation constant of the ligand-receptor complex and the maximum concentration of the binding centers were determined. It was shown that the tricyclic antidepressant imipramine inhibits the binding of 3H-5-HT with IC50 = 280 nm. The binding of 3H-5-HT with lymphocytes is Na-insensitive but is inhibited by Ca and Mg ions. The interrelation of 3H-5-HT with the lymphocytes differs in nature from the process of reuptake capture of 3H-5-HT by the blood platelets.