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Related Experiment Videos

Cell-substrate separation: effect of applied force and temperature

C M Lo1, M Glogauer, M Rossi

  • 1Medical Research Council Group in Periodontal Physiology, University of Toronto, Ontario, Canada.

European Biophysics Journal : EBJ
|February 17, 1998
PubMed
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Researchers used Electric Cell-substrate Impedance Sensing (ECIS) and magnetic beads to measure how cell-substrate separation changes under force. This study quantifies cell adhesion forces and their temperature dependence.

Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Understanding cell-substrate interactions is crucial for tissue engineering and cell-based assays.
  • Quantifying the mechanical properties of cell adhesion provides insights into cellular behavior and responses to physical stimuli.

Purpose of the Study:

  • To measure the change in cell-substrate separation under controlled force using a novel combination of techniques.
  • To determine the junctional resistivity and cell-substrate distance.
  • To investigate the temperature dependence of cell adhesion forces and properties.

Main Methods:

  • Combined Electric Cell-substrate Impedance Sensing (ECIS) with collagen-coated magnetic beads to apply controlled force (320 or 560 pN) to the dorsal surface of cells.
  • Used integrin receptors on osteoblastlike ROS 17/2.8 cells for bead attachment.

Related Experiment Videos

  • Analyzed impedance data to calculate cell-substrate separation and junctional resistivity at different temperatures (4, 22, and 37 degrees C).

    • Main Results:

    • Applied forces increased cell-substrate separation by 10-25%.
    • Measured cell-substrate distances decreased with increasing temperature (84 nm at 4°C, 45 nm at 22°C, 38 nm at 37°C).
    • Calculated cell-substrate spring constants of approximately 28 pN/nm at 22°C and 63 pN/nm at 37°C.
    • Estimated individual integrin-ligand adhesion bond spring constants between 10^-3 and 10^-1 pN/nm.
    • Observed an increase in the number of adhesion bonds per cell with increasing temperature.

    Conclusions:

    • The applied forces induce significant changes in cell-substrate separation, demonstrating the mechanical responsiveness of cell adhesions.
    • Cell-substrate adhesion strength and the number of adhesion bonds increase with temperature.
    • The developed method provides a quantitative approach to study cell mechanics and adhesion properties.