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Step-gradient capillary electrochromatography
M R Euerby1, D Gilligan, C M Johnson
1Pharmaceutical and Analytical R & D, Astra Charnwood, Loughborough, Leicestershire, UK.
The Analyst
|February 17, 1998
Abstract:
The analytical benefits of using a step-gradient in capillary electrochromatography (CEC) are demonstrated. The application of step-gradient CEC to the analysis of six diuretics of widely differing lipophilicities was evaluated and shown to result in a marked reduction in the analysis time and an improvement in the peak shape for later-eluting lipophilic components. When the step-gradient approach was performed in an automated mode, the retention time RSD for repeated injections was below 1%.