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Related Experiment Videos

No turning, a mouse mutation causing left-right and axial patterning defects

P G Melloy1, J L Ewart, M F Cohen

  • 1Department of Biology, University of Pennsylvania, Philadelphia 19104, USA.

Developmental Biology
|February 19, 1998
PubMed
Summary
This summary is machine-generated.

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A new mouse mutation, no turning (nt), causes severe developmental defects including left/right patterning errors. This study reveals the notochord

Area of Science:

  • Developmental biology
  • Genetics
  • Embryology

Background:

  • Left/right axis patterning is crucial for organ asymmetry and organism viability.
  • Genes involved in left/right patterning often also regulate anteroposterior and dorsoventral patterning.

Purpose of the Study:

  • To investigate a novel mouse mutation affecting embryonic turning and axial patterning.
  • To elucidate the role of the notochord in left/right axis determination.

Main Methods:

  • Characterization of a spontaneous recessive lethal mouse mutation, no turning (nt).
  • Histological analysis and gene expression studies (sonic hedgehog, Brachyury, HNF-3 beta).
  • Examination of Nodal and lefty gene expression patterns.

Main Results:

Related Experiment Videos

  • The nt mutation causes defects in embryonic turning, neural tube closure, somite formation, and caudal truncation.
  • Mutant embryos display randomized heart looping and cardiovascular abnormalities.
  • Nodal and lefty expression is altered, and the notochord degenerates during development.

Conclusions:

  • The notochord plays an active role in left/right patterning.
  • The nt mutation may affect left/right patterning by modulating notochordal HNF-3 beta expression.