Analysis of electron microscope autoradiographs using the hypothetical grain analysis method

Cross-fire between adjacent labelled sources can often introduce problems in the analysis of electron microscope autoradiographs. A general method is described which makes allowance for this cross-fire and is then applied to the analysis of autoradiographs of 3H-proline in the accessory olfactory bulb of the mouse. It is shown how the method allows estimates to be made of the radioactivity of the various cell types and the accuracy of these estimates and the errors in obtaining them are discussed.