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Evaluation of a noninstrumented disposable method for quantifying serum theophylline concentrations
M J Asmus1, G Milavetz, M E Teresi
1Division of Clinical and Administrative Pharmacy, College of Pharmacy, University of Iowa, Iowa City 52242-1112, USA.
Pharmacotherapy
|February 20, 1998
Summary
The new AccuMeter point-of-care theophylline assay shows good precision and minimal bias compared to standard laboratory and other point-of-care methods. Sample collection type did not impact the AccuMeter
Area of Science:
- Clinical Chemistry
- Point-of-Care Testing
- Pharmacokinetics
Background:
- Theophylline is a critical medication for managing asthma.
- Accurate monitoring of theophylline levels is essential for therapeutic efficacy and safety.
- Point-of-care (POC) assays offer rapid theophylline concentration results.
Purpose of the Study:
- To evaluate the performance of a novel POC theophylline assay, AccuMeter.
- To compare AccuMeter's accuracy and precision against a standard laboratory assay (TDx) and another POC method (AccuLevel).
Main Methods:
- Prospective study involving 40 asthma patients on maintenance theophylline therapy.
- Theophylline concentrations were measured using AccuMeter, TDx, and AccuLevel assays.
- Performance was assessed by comparing accuracy (bias) and precision (mean squared error).
Main Results:
- AccuMeter demonstrated good precision and minimal bias when compared to the TDx assay across different sample types (capillary, serum, heparinized).
- The method of sample collection did not significantly affect AccuMeter's accuracy.
- Relative bias and precision between AccuMeter and AccuLevel were also evaluated.
Conclusions:
- The AccuMeter assay is a reliable POC method for theophylline monitoring.
- Its performance is comparable to established laboratory and POC methods.
- AccuMeter offers a viable option for rapid theophylline level determination in clinical settings.
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