[Determination of antibodies to streptolysin O]

A new procedure for the assessment of the antibody against streptolysin O by a micromethod was tested by comparison with the photometric reference method. Agreement between the two methods was very good. The differences in titres did not exceed the titration error of the method. Based on the tested micromethod, a diagnostic set was produced in the Czech Republic which is to be used for common titration of antistreptolysin O in serum. The ITEST ASO was tested in relation to the photometric reference method and standardized neutralization micromethod of the National reference laboratory for streptococci and enterococci in Prague. From a group of 135 selected sera with different titres there was agreement between all tested methods. Selected kits used abroad for the titration of antistreptolysin O were tested in the National reference laboratory by using the authors own standardized materials and methods.