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[Biparametric analysis of the apoptotic index and the proliferation index (KI-67) in solid tumors]
F Mangili1, C Cigala, C Gattuso
1Servizio di Anatomia e Istologia Patologica, Istituto Scientifico H San Raffaele, Milano.
Introduction:
Cell kinetic data are important indicators of the aggressiveness of tumor and treatment response. In this study biparametric analysis of proliferation and apoptotic index was performed on paraffin-embedded tissue from certain neoplasia.
Material And Methods:
We studied 107 cases of neoplastic and non neoplastic lesions. For each case, on the same section, TUNEL reaction and immunohistochemical protein Ki67 expression analysis were performed.
Results:
On 102 out of 107 cases (95.3%) we obtained valid results both about apoptotic and proliferative indices.
Conclusions:
This method could supply, at the same time, interesting information about the two possible different patterns of neoplastic growth: one related to an increase fraction of proliferating cells, the other to decrease in apoptotic cell death. Cell kinetic data may have interesting implications on therapeutic choice.